Name
Histone H1.4(Thr18) Antibody, FITC Conjugated
Size
0.1ml
Catalog number
bs-3171R-FITC
Price
350 EUR
More details
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Long name
Histone H1.4(Thr18) Polyclonal Antibody, FITC Conjugated
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Category
Conjugated Primary Antibodies
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Conjugation
FITC
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Host Organism
Rabbit (Oryctolagus cuniculus)
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Target Antigen
Histone H1.4 Thr18
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Specificity
This is a highly specific antibody against Histone H1.4 Thr18. This phosphorylation site is found in Rat H1C as well, but not Human or Mouse H1C. This phosphorylation site is homologous to Thr18 in Rat H1C/H1D and Mouse H1D. Due to the highly conserved nature of these histones, this antibody may react with these members when phosphorylated at this site.
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Modification
Phosphorylation
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Modification site
Thr18
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Clonality
Polyclonal
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Clone
Polyclonal antibody
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Concentration
1ug per 1ul
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Immunogen_range
2-30/219
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Subcellular location
Nucleus
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Source
KLH conjugated synthetic phosphopeptide derived from human Histone H1.4 around the phosphorylation site of Thr18
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Gene ID number
3008
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Swiss Prot
P10412
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Tested applications
IF(IHC-P)
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Recommended dilutions
IF(IHC-P)(1:50-200)
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Crossreactivity
Human, Mouse, Rat
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Crossreactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Antigen background
Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).
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Purification method
This antibody was purified via Protein A.
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Storage conditions
Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
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Excitation Emission
494nm/518nm
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Synonyms
H1E; H1.4; H1F4; H1s-4; dJ221C16.5; Histone H1.4; Histone H1b; Histone H1s-4; HIST1H1E
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Properties
If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.
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Additional conjugation
Anti-FITC Antibody
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French translation
anticorps