Name
Histone H1 (Lys52) Antibody, Cy3 Conjugated
Size
0.1ml
Catalog number
bs-0931R-Cy3
Price
368 EUR
More details
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Long name
Histone H1 (Lys52) Polyclonal Antibody, Cy3 Conjugated
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Category
Conjugated Primary Antibodies
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Conjugation
Cy3
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Host Organism
Rabbit (Oryctolagus cuniculus)
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Target Antigen
Acetyl-Histone H1 Lys52
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Specificity
This is a highly specific antibody against Acetyl-Histone H1 Lys52. This antibody will react with members of the Histone H1 family that have an acetylated lysine positioned in the motif AS(Ac-K)ER. This include H1.1, H1.2, H1.3, H1.4, and H1.5.
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Modification
Acetylation
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Modification site
Lys52
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Clonality
Polyclonal
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Clone
Polyclonal antibody
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Concentration
1ug per 1ul
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Immunogen_range
30-60/219
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Subcellular location
Nucleus
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Source
KLH conjugated synthetic acetylpeptide derived from human Histone H1.4 around acetylation site of Lys52
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Gene ID number
3008
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Swiss Prot
P10412
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Tested applications
IF(IHC-P)
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Recommended dilutions
IF(IHC-P)(1:50-200)
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Crossreactivity
Human, Mouse, Rat
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Crossreactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Antigen background
Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).
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Purification method
This antibody was purified via Protein A.
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Storage conditions
Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
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Excitation Emission
512,550nm/570,615nm
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Synonyms
H1E; H1.4; H1F4; H1s-4; dJ221C16.5; Histone H1.4; Histone H1b; Histone H1s-4; HIST1H1E
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Properties
If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser. 1 of the Anti-Acetyl-Histone H1 Lys52 can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC. This Bioss Primary Conjugated Antibodies antibody is well suited for fluorescent microscopy.
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Additional conjugation
cy3 conjugation kit
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French translation
anticorps