![Mouse Monoclonal [clone AE-4] (IgG2a) to Human Histone H1 Antibody](/images/halftone/products/big/0.jpg)
![Mouse Monoclonal [clone AE-4] (IgG2a) to Human Histone H1 Antibody](/images/halftone/products/big/17.jpg)
![Mouse Monoclonal [clone AE-4] (IgG2a) to Human Histone H1 Antibody](/images/halftone/products/big/1.jpg)
Name
Mouse Monoclonal [clone AE-4] (IgG2a) to Human Histone H1 Antibody
Size
50ug
Catalog number
GEN246041
Price
597 EUR
More details
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Also known as
Histone H1
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Other names
histone H1; N/A; histone H1; N/A; N/A
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
H1; N/A; leH1; N/A
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Gene name synonims
H1; N/A; leH1; N/A
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Other gene names
H1; N/A; leH1; N/A
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG2a
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Clone
AE-4
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Host organism
Mouse (Mus musculus)
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Species reactivity
Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross-reactivity
Recognizes histone H1. The histone proteins serve as a structural scaffold for the organization of nuclear DNA into chromatin. In eukaryotic cells there are 4 core histone proteins, H2A, H2B, H3 and H4 that assemble to form an octamer round which the DNA is wrapped in a sequence dependant manner. The resulting complex is known as a nucleosome and is the basic subunit of chromatin. Histone H1 acts as a linker and binds to each nucleosome, and by self affinity, links these nucleosomes together resulting in the 30 nm chromatin fiber. In blots of rat brain homogenate a duplet is observed at 27-29 kD consistent with Histone H1. Speckled staining is observed in the nucleus of normal and malignant cells.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
Protein G Purified
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Form/Appearance
PBS, 0.09% sodium azide.
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Concentration
1 miligram per 1 mililiter
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Storage and shipping
For short term storage keep the antibody at +4 degrees Celsius. For longer storage the antibody should be kept at -20 degrees Celsius. Cycles of freezing and defrosting may denaturate the polypeptide chains of the antibodies and reduce their activity.
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Tested applications:
Immunohistochemistry (IHC - Paraffin; IHC - Frozen), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
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Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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French translation
anticorps