Histone H1.3/H1.4 (phospho-T17) Rabbit monoclonal antibody
Histone H1.4; Histone H1b; Histone H1s-4; HIST1H1E; H1F4; Histone H1.3; Histone H1c; Histone H1s-2; HIST1H1D; H1F3
WB ICC/IF IHC
WB: 1:500-1:1000 IHC/ICC/IF: 1:50-1:200
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Protein A affinity purified
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
This antibody detects endogenous levels of Histone H1.3 or H1.4 protein only when pho17.
Western blot (WB) analysis of Histone H1.3/H1.4 (phospho-T17) Rabbit mAb at 1:500 dilution Lane 1: Untreated CRC whole cell lysates Lane2: CRC cells treated with 1.5µg/ml Colcemid for 12 hours whole cell lysates
~ 30 kDa
For research use only, not for use in diagnostic procedure.
If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.Rabbits are used for polyclonal antibody production by Bioworld. Rabbit antibodies are very stable and can be stored for several days at room temperature. Bioworld adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.