H1, histone H1
Available ordering format
native H1 protein purified from Nicotiana tabaccum (H1A, H1B H1C,D,E,F)
Affinity purified serum
How to reconstitute
For reconstitution add 50 µl of sterile water.
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
western blot (WB), immunocytochemistry (ICC)
AS10 710 | H3 | histone H3, rabbit antibody AS11 1753 | HDT3 | histone deacetylase, rabbit antibody collection of antibodies to DNA/RNA/cell cyclePlant protein extraction buffer Secondary antibodies
Recommended dilutions for use
1 : 5000 with standard ECL (WB), 1: 100 - 1: 500 (ICC)
Molecular weight (expected | аpparent)
15 | 17 kDa
Arabidopsis thaliana, Nicotiana tabacum, Triticum aestivum
Lathyrus sativus, Phaseolus vulgaris, Pisum sativum, Solanum lycopersicum, Vicia faba
no confirmed exceptions from predicted reactivity known in the moment
Protocol for isolation of cytosolic and nuclear fractions can be found here.Protocol for immunostatining in whole-mount plant tissues: An efficient method for quantitative, single-cell analysis of chromatin modification and nuclear architecture in whole-mount ovules in Arabidopsis Wenjing She, Daniel Grimanelli, Célia Baroux Journal of Vizualized Experiments (JoVE), in press
She and Baroux (2015). Chromatin dynamics in Pollen Mother Cells underpin a common scenario at the somatic-to-reproductive fate transition of both the male and female lineages in Arabidopsis. Front. Plant Sci. | doi: 10.3389/fpls.2015.00294. She et al. (2013). Chromatin reprogramming during the somatic to-reproductive cell fate transition in plants. Development Oct;140(19):4008-19. doi: 10.1242/dev.095034. Epub 2013 Sep 4. (Arabidopsis thaliana, immunostaining)
Histone 1 (H1) is a protein located in nuclei, incorporated into chromatin.
Refer to NCBI
Refer to NCBI