Name
H1, histone H1
Size
50 µg
Catalog number
AS11 1801
Price
434 EUR
More details
-
Available ordering format
Lyophilized
-
Immunogen
native H1 protein purified from Nicotiana tabaccum (H1A, H1B H1C,D,E,F)
-
Raised in
Rabbit
-
Clonality
Polyclonal
-
Clone
Polyclonal
-
Purification
Affinity purified serum
-
How to reconstitute
For reconstitution add 50 µl of sterile water.
-
Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
-
Verified applications
western blot (WB), immunocytochemistry (ICC)
-
Connected products
AS10 710 | H3 | histone H3, rabbit antibody AS11 1753 | HDT3 | histone deacetylase, rabbit antibody collection of antibodies to DNA/RNA/cell cyclePlant protein extraction buffer Secondary antibodies
-
Recommended dilutions for use
1 : 5000 with standard ECL (WB), 1: 100 - 1: 500 (ICC)
-
Molecular weight (expected | аpparent)
15 | 17 kDa
-
Verified reactivity
Arabidopsis thaliana, Nicotiana tabacum, Triticum aestivum
-
Possible reactivity
Lathyrus sativus, Phaseolus vulgaris, Pisum sativum, Solanum lycopersicum, Vicia faba
-
No reactivity
no confirmed exceptions from predicted reactivity known in the moment
-
Supplementary information
Protocol for isolation of cytosolic and nuclear fractions can be found here.Protocol for immunostatining in whole-mount plant tissues: An efficient method for quantitative, single-cell analysis of chromatin modification and nuclear architecture in whole-mount ovules in Arabidopsis Wenjing She, Daniel Grimanelli, Célia Baroux Journal of Vizualized Experiments (JoVE), in press
-
References
She and Baroux (2015). Chromatin dynamics in Pollen Mother Cells underpin a common scenario at the somatic-to-reproductive fate transition of both the male and female lineages in Arabidopsis. Front. Plant Sci. | doi: 10.3389/fpls.2015.00294. She et al. (2013). Chromatin reprogramming during the somatic to-reproductive cell fate transition in plants. Development Oct;140(19):4008-19. doi: 10.1242/dev.095034. Epub 2013 Sep 4. (Arabidopsis thaliana, immunostaining)
-
Scientific context
Histone 1 (H1) is a protein located in nuclei, incorporated into chromatin.
-
Notes
None
-
Protein number
Refer to NCBI
-
TAIR number
Refer to NCBI