Name
ASH1, Histone-lysine N-methyltransferase ASH1
Size
50 µg
Catalog number
AS14 2816
Price
434 EUR
More details
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Available ordering format
Lyophilized in PBS pH 7.4
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Immunogen
N-terminal GST-fusion of the peptide containing amino acids 1756-1855 of the Drosophila melanogaster Ash1protein, UniProt: Q9VW15
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified serum
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How to reconstitute
For reconstitution add 50 µl of sterile water.
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB), immunolocalization (IL), chromatin immunoprecipitation (ChIP)
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Connected products
Secondary antibodies
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Recommended dilutions for use
1: 1000 (WB), 1:500 (IL), 10ng/ul (ChIP)
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Molecular weight (expected | аpparent)
236 | 300 kDa
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Verified reactivity
Drosophila melanogaster
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Possible reactivity
Drosophila melanogaster
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No reactivity
no confirmed exceptions from predicted reactivity are currently known
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Supplementary information
to be added when available
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References
Kahn et al. (2016). Interdependence of PRC1 and PRC2 for recruitment to Polycomb Response Elements. Nucleic Acids Res. 2016 Aug 23. pii: gkw701. [Epub ahead of print]. Lee et al. (2015). Genome-wide activities of Polycomb complexes control pervasive transcription. Genome Res. 2015 Aug;25(8):1170-81. doi: 10.1101/gr.188920.114. Epub 2015 May 18.
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Scientific context
Histone-lysine N-methyltransferase ash1 is a Trithorax group (TrxG) protein that has histone methyltransferase activity. Specifically trimethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation.
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Notes
None
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Protein number
Q9VW15
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TAIR number
Refer to NCBI