Anti-Histone H1.4 (Polyclonal), ALEXA Fluor 594
Conjugated Primary Antibody
ALEXA FLUOR® 594
Rabbit (Oryctolagus cuniculus)
This antibody reacts specifically with Histone H1.4
No modification has been applied to this antibody
1ug per 1ul
KLH conjugated synthetic peptide derived from human Histone H1.4
Applications with corresponding dilutions
Cross reactive species
Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
Cross Reactive Species details
No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
Histone H1b are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
Purified by Protein A.
Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
HIST1H1E; H1F4; Histone H1.4; Histone H1b; H14_HUMAN; Histone H1s-4.
Also known as
Histone H1.4 Polyclonal Antibody
Anti-Histone H1.4 Polyclonal
Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
For facs or microscopy Alexa 1 conjugate.
Alexa Fluor,ALEXA FLUOR® 594
Alexa conjugate 1
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.