HDAC6 (H1203) polyclonal antibody
Histone deacetylase 6; HD6; HDAC 6; KIAA0901; JM21
Synthetic peptide, corresponding to the C-terminual of Human HDAC6.
WB IHC IF
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is> 95% (by SDS-PAGE).
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
HDAC6 (H1203) polyclonal antibody detects endogenous levels of HDAC6 protein.
Western blot (WB) analysis of HDAC6 (H1203) pAb at 1:500 dilution Lane1:PC12 whole cell lysate(40ug) Lane2:BV2 whole cell lysate(40ug) Lane3:A375 whole cell lysate(40ug) Lane4:MCF-7 whole cell lysate(40ug)
~ 131, 160 kDa
For research use only, not for use in diagnostic procedure.
If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.